题 目：西班牙柑橘产业及其病毒病研究现状
时 间：2026年9月28日（星期一）14:30
主讲人：Antonio Olmos、Ana Belén Ruiz García
地 点：柑桔研究所综合楼六楼会议室
主办单位：柑桔研究所
主讲人简介：Antonio Olmos博士，西班牙瓦伦西亚农业研究院（IVIA）高级研究员，主管木本作物病毒的研究工作，聚焦于植物病毒诊断、分子特征分析、流行病学研究、高通量测序（HTS）技术，以及病毒性病害监测与防控策略的制定。作为西班牙农业、渔业与食品部下属的木本植物、马铃薯、甘薯及草莓病毒国家参考实验室的负责人，他还积极参与国际植物健康事务，包括欧洲和地中海植物保护组织（EPPO）病毒学与植原体专家小组的工作，并担任Journal of Plant Pathology副主编。他的研究成果推动了分子诊断技术的发展，并助力新发及此前未被描述的植物病毒鉴定与特性解析，尤其关注柑橘、葡萄、核果类果树及橄榄等作物。目前，他的研究重点包括新发柑橘病毒的监测与流行病学研究，特别是针对柑橘黄化脉明病毒（CYVCV）的相关研究。 Ana Belén Ruiz-García博士，西班牙瓦伦西亚农业研究院（IVIA）研究员，专注于植物病毒学及木本作物病毒病研究。其研究重点涵盖分子诊断、病毒特性鉴定、流行病学、基因组学，以及高通量测序（HTS）技术在植物病毒组及新发病毒病研究中的应用。她担任西班牙农业、渔业与食品部下属的木本植物、马铃薯、甘薯及草莓病毒国家参考实验室副主任，积极参与遵循国际标准的病毒、类病毒及植原体分子诊断方法的实施、验证和认证工作，并致力于将统一的诊断方案应用于官方监测及植物健康项目中。其科研工作涉及柑橘、葡萄、橄榄和其他木本植物病毒研究，尤其关注新发病毒及先前未描述病毒的鉴定与基因组特征解析，同时开发用于检测与监测病毒的分子工具。此外，她的研究还探索了真菌病毒的鉴定，以及具有潜在抗菌活性病毒蛋白的特性分析，从而将植物病毒学的研究拓展至新型生物活性分子及生防制剂领域。
讲座简介：
Citrus in Spain: Production, Research and Plant Health Challenges (Dr. Antonio Olmos)
Spain is the leading citrus-producing country in the European Union and one of the world’s major exporters of fresh citrus fruit. Production is concentrated along the Mediterranean coast, with the Valencian Community representing the historical core of Spanish citriculture and a major production area for mandarins and sweet oranges.
This seminar will provide an overview of Spanish citriculture, including its production, main citrus species and cultivars, and the major plant health challenges affecting the sector. The role of the Valencian Institute for Agricultural Research (IVIA) in citrus research will be presented, with particular emphasis on plant virology. Our research group specializes in the diagnosis, molecular characterization and epidemiology of viruses and viroids affecting woody crops and hosts the Spanish National Reference Laboratory for viruses of woody plants of the Ministry of Agriculture, Fisheries and Food.
A major focus of our current research is the integration of molecular diagnostics with high-throughput sequencing (HTS) and genomic surveillance. In the last two years, this approach has revealed previously unreported components of the Spanish citrus virome, including Citrus yellow vein clearing virus (CYVCV), CTV genotypes RB and T3 and citrus virus A (CiVA). These findings have enabled the development of new diagnostic tools and have opened new studies on virus diversity, epidemiology and emerging disease risks. 2. Citrus Yellow Vein Clearing Virus in Spain: Emergence, Diagnosis and Epidemiology (Dr. Ana Belén Ruiz García)
Citrus yellow vein clearing virus (CYVCV) is the causal agent of citrus yellow vein clearing disease and an emerging threat to citrus production. Originally associated mainly with Asia, its recent detection in Italy and Spain has raised concern about its establishment and spread in the Mediterranean citrus industry.
This seminar will present the emergence of CYVCV in Spain and the diagnostic, genomic and epidemiological investigations undertaken following its detection through HTS-based surveillance. Subsequent studies have confirmed infections in different citrus hosts, including both symptomatic and asymptomatic plants. Genome diversity and phylogenetic studies have revealed the existence of a new group phylogenetically distant from the previous described ones. Symptom expression, viral distribution in different plant tissues and impact on the main Spanish cultivars/roostoks are under investigation.
A highly sensitive real-time RT-qPCR assay for the accurate detection of CYVCV has been developed and validated according to EPPO standards and subsequently adapted to digital PCR and portable PCR platforms. The method has been applied to develop sampling strategies, that have turned out to be a key step in CYVCV diagnosis.
Ongoing genomic and epidemiological studies are investigating CYVCV diversity in the EPPO region, transmission, host range, alternative hosts, citrus susceptibility and host-virus interactions. The Spanish experience illustrates how HTS, molecular diagnostics, optimized sampling and genomic epidemiology can be combined to respond to an emerging citrus virus and support improved surveillance and disease-management strategies.
